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Image Search Results
Journal: Science Advances
Article Title: Integration of reconfigurable microchannels into aligned three-dimensional neural networks for spatially controllable neuromodulation
doi: 10.1126/sciadv.adf0925
Figure Lengend Snippet: ( A ) Schematic illustration depicting microfluidic neuromodulation with KCl via the center channel to an aligned 3D neural network. ( B ) Computational simulation of 3D transient diffusion of K + (39.0983 Da) into a collagen scaffold at 239.7 s. ( C ) Color-mapped differential confocal fluorescence images (left images in each panel) of Fluo-4 AM (Ca 2+ signal) between two adjacent time frames [ I Fluo-4 AM, t ( i ) − I Fluo-4 AM, t ( i −1) ] and snapshots of the computational calculation (right images in each panel) from the solutions in (B) at 0 ( t 0 ), 28.2 ( t 1 ), 51.7 ( t 2 ), and 239.7 ( t 3 ) s. Scale bar, 500 μm. ( D ) y -Mean concentration of K + along the x axis at t i . ( E ) [ I Fluo-4 AM, t ( i ) − I Fluo-4 AM, t ( i −1) ] along the x axis. Light and bold colors represent y -mean intensities and fitted curves generated by a smoothing option of LOWESS in Prism.
Article Snippet: We generated fitted curves using a smoothing option of
Techniques: Diffusion-based Assay, Fluorescence, Concentration Assay, Generated
Journal: Science Advances
Article Title: Integration of reconfigurable microchannels into aligned three-dimensional neural networks for spatially controllable neuromodulation
doi: 10.1126/sciadv.adf0925
Figure Lengend Snippet: ( A ) Schematic illustration depicting spatiotemporally resolved microfluidic neuromodulation by two consecutive pulsatile deliveries of KCl via the left microchannel to an aligned 3D neural network. ( B ) Color-mapped differential confocal fluorescence images of Fluo-4 AM (Ca 2+ signal) between two adjacent representative time frames [ I Fluo-4 AM, t ( i ) − I Fluo-4 AM, t ( i -1) ] (a) and [ I Fluo-4 AM, t ( i ) − I Fluo-4 AM, t ( i −1) ] along the x axis after the first pulse: −18 ( t 0 ), 0 ( t 1 ), 6 ( t 2 ), and 66 ( t 3 ) s and the second pulse: 522 ( t 0 ), 525 ( t 1 ), 570 ( t 2 ), and 603 ( t 3 ) s (b). Scale bars, 500 μm. Curve profiles represent y -mean intensities generated by a smoothing option of LOWESS in Prism.
Article Snippet: We generated fitted curves using a smoothing option of
Techniques: Fluorescence, Generated
Journal: Science Advances
Article Title: Integration of reconfigurable microchannels into aligned three-dimensional neural networks for spatially controllable neuromodulation
doi: 10.1126/sciadv.adf0925
Figure Lengend Snippet: ( A and B ) Schematic illustrations depicting spatially resolved microfluidic neuromodulation with KCl via the right microchannel and TTX, nifedipine, mibefradil via the center microchannel to an aligned 3D neural network. ( C ) Color-mapped differential confocal fluorescence images of Fluo-4 AM (Ca 2+ signal) between two adjacent representative time frames [ I Fluo-4 AM, t ( i ) − I Fluo-4 AM, t ( i −1) ] (a) and [ I Fluo-4 AM, t ( i ) − I Fluo-4 AM, t ( i −1) ] along the x axis upon simultaneous treatments of TTX: −6 ( t 0 ), 0 ( t 1 ), 78 ( t 2 ), and 210 ( t 3 ) s; nifedipine: −3 ( t 0 ), 0 ( t 1 ), 39 ( t 2 ), and 72 ( t 3 ) s; and mibefradil: −6 ( t 0 ), 0 ( t 1 ), 21 ( t 2 ), and 54 ( t 3 ) s (b). Scale bars, 500 μm. Curve profiles represent y -mean intensities generated by a smoothing option of LOWESS in Prism.
Article Snippet: We generated fitted curves using a smoothing option of
Techniques: Fluorescence, Generated